Request QuoteCatalog Number: xP529456TPRSize: 0.2-1mg

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Recombinant Alanine--tRNA ligase (alaS)

Recombinant Alanine--tRNA ligase (alaS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529456TPRYeast1mgQuote
EP529456TPRE. coli1mgQuote
BP529456TPRBaculovirus200ugQuote
MP529456TPRMammalian Cell200ugQuote

Protein Information

SpeciesTreponema pallidum (strain Nichols)
UniProt IDO83980
Gene NamealaS; Locus:TP_1017
Protein NameAlanine--tRNA ligase
Region Expressed1-605
Expression Tag6xHis
Purity>90%
AA SequenceMSIPIRADQLRSRYLAFFSQKAHVVISGKSLVPEHDPTVLFTTAGMHPLVPYLMGEPHPA GTRLVNAQKCLRTGDIDAVGDNSHLTFFEMLGNWSLGDYFKEEAIAFSFEFLTGAPWLGI SPDRLSVTVFAGDEAVARDEESAAIWERLGIARTHIHFLPRADNWWGPTGETGPCGPDTE IFFDTGVPPCSVSCRPGCSCGKYVEIWNDVFMQYRKDADGRYRPLERYCVDTGMGIERTV AVLQGKRSVYDTEIFTPLLERIGQLCGKRYGCQGAHDVSMRIVCDHIRAATFILGDPVPV RPSNVGAGYVLRRIIRRSVRHGRKLGIDGEFLSSLARVVIGQYAAVYPELEEKATCIAQE LANEERKFLDALRKGEAEYERMIPKFLQGTEREIPGSVAFRLYDTYGFPLELTEELARES GLRVDRAGFDTAFQAHQACSRIGAQRVFKGGLADHSAETTAYHTATHLLHQALRVVLGTH VQQKGSNITAERLRFDFSHPRPMSAQEKVQVEQLVNEQIRADLPVCCEVMSLEDAMNSGA VALFGEKYESTVKVYSIGTFSREVCGGPHVARTGQLGRFSIQKEQSSAAGVRRIRAILEK SGEKS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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