$148.20Catalog Number: PYT613-5ugSize: 5ug

aFGF Bovine

Fibroblast Growth Factor Acidic Bovine

Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. (See Jaye et al). The FGF acidic is purified by proprietary chromatographic techniques.

Accession

P03968

Biological Activity

Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF and 0ug/ml heparin.

Formulation

Each 5ug aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 90%.

Solubility

It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.

Source

Bovine Brain.

Stability

Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18oC. Upon reconstitution aFGF should be stored at 4oC between 2-3 weeks and for future use below
-18oC.
Please prevent freeze-thaw cycles.

Synonyms

HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.

Quantity

$148.20

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