Request QuoteCatalog Number: xP522120SXVSize: 0.2-1mg

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Recombinant AdoMet-dependent rRNA methyltransferase spb1 (spb1)

Recombinant AdoMet-dependent rRNA methyltransferase spb1 (spb1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP522120SXVYeast1mgQuote
EP522120SXVE. coli1mgQuote
BP522120SXVBaculovirus200ugQuote
MP522120SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO42832
Gene Namespb1; aka: pmt2; ORFs:SPAC1687.11
Protein NameAdoMet-dependent rRNA methyltransferase spb1
Region Expressed1-802
Expression Tag6xHis
Purity>90%
AA SequenceMGKSQKKTAKGRLDKWYKLAKEQGYRSRAAFKLVQLNQKYSFLEKAKVIIDLCAAPGGWL QVASKTCKPGSLIVGVDLAPIKPIPNCHTFVEDITSDKCRSQLRGYLKTWKADVVLHDGA PNVGSAWLQDAYGQAQLVLMSMKLACEFLVAGGTFVTKVFRSRDYNNLLWVFKQLFNKVE ATKPPSSRNVSAEIFVVCRGYKAPKKLDPRFTDPRTVFEEVQEPVTNVDAKVFHPEKRKR SREGYADDDYTLHKTVLASEFVTANDPIQILGTSAEIVFPKDDEECQRLYNLDVTTEEIL LCCSDLQVLGKKEFRDILRWRLKIRDEMGIGKKVEDEQKTVVEEIPEMDEEERLDQELQD LSEAERVKLKRERRKANQRKQREIVRMQMGMLAPMDIGLEHEAMGEDSLFGLATAEKHGL KELENGTLPVTESVDEEVSTDNEVEYDSDDERDRLEADLDSMYSDYTKRKAESDVKYRVK KARGDLDDEEWNGIDNGTESDDSQIAETNFATPDKDRLTTSLLDKGSTKDGLSRKARMFF DQDIFDGIEDADADVEIMSMNRAAIKKREAELASQNNDDGSKGDQSEDSNDHIEVVPVAS AHDEDDDWNSDSDNDENNVEIVTAEAMTLAQDIASRRKSKADLIDEGYNRWSFQSKEGLP DWFLDEETTVNKPNKPITKEAVLALREKMKALNARPIKKVLEAQGRKKMRTIKRLQRVAK KAEGISESGDMTESEKAKEISRLVSRATKSKPKAKPTLVVAKGPNKGLKSRPKGVKGKYK MVDSRMKKDLRAQKRLAKKGRR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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