Activin-A Human Recombinant, Active
Active form Activin-A Human Recombinant produced in Plant is a single homodimeric, glycosylated, polypeptide chain containing 2 x 116 amino acids and having a molecular weight of 27.4kDa.
The Active form Activin-A is fused to a 6-His tag at N-terminus and purified by standard chromatographic techniques.
Accession
Amino Acid Sequence
HHHHHHGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSG
YHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFA
NLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a specific activity of 2MIU/mg.
Formulation
Active form Activin-A was lyophilized from a concentrated 1mg/ml protein solution containing 50mM Tris-HCl pH-7.4
Physical Appearence
Lyophilized freeze dried powder.
Purity
Greater than 98% as obsereved by SDS-PAGE.
Solubility
INHBA protein should be reconstituted in distilled water to a concentration of 50 ug /ml. Due to the protein nature, dimmers and multimers may be observed.
Source
Stability
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.
Synonyms
Inhba, Inhibin beta A, FSH releasing protein.
