$1,710.00Catalog Number: PYT057-100ugSize: 100ug

Activin B Human Plant-Active

Activin-B Human Recombinant Active

Activin B human Recombinant produced in Nicotiana benthamiana plant is a single chain containing 123 amino acids (molecular formula C615H910N178O177S12).
Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

Accession

P09529

Amino acid sequence

HHHHHHHHHH GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECG

Biological Activity

The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation.
<5ng/mL are required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Formulation

Lyophilized from 1mg/ml solution in Tris HCl 0.05M buffer at pH 7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by Analysis by SDS-PAGE.

Serological Identification

The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electro blotting to a NC membrane and visualized by immune-detection with specific Activin B antibody.

Solubility

It is recommended to reconstitute the lyophilized Activin B in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Nicotiana benthamiana plant

Stability

Lyophilized Activin B although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Activin B should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Inhibin beta B (activin AB beta polypeptide), Inhibin, beta-2, Activin beta-B chain, MGC157939.

Quantity

$1,710.00

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