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Recombinant 72 kDa type IV collagenase (Mmp2)

Recombinant 72 kDa type IV collagenase (Mmp2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP014666RAYeast1mgYP014666Ra
EP014666RAE. coli1mgQuote
BP014666RABaculovirus200ugQuote
MP014666RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDP33436
Gene NameMmp2
Protein Name72 kDa type IV collagenase
Region Expressed445-662
Expression Tag6xHis
Purity>90%
AA SequenceYGPSPDADTDTGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDK PTGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWVYSASTLERGYPKPLTSLGLP PDVQQVDAAFNWSKNKKTYIFSGDKFWRYNEVKKKMDPGFPKLIADSWNAIPDNLDAVVD LQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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