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Recombinant 60 kDa chaperonin (groL)

Recombinant 60 kDa chaperonin (groL) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP528985EKRYeast1mgQuote
EP528985EKRE. coli1mgQuote
BP528985EKRBaculovirus200ugQuote
MP528985EKRMammalian Cell200ugQuote

Protein Information

SpeciesEnterobacter asburiae
UniProt IDO66190
Gene NamegroL; aka: groEL, mopA
Protein Name60 kDa chaperonin
Region Expressed1-539
Expression Tag6xHis
Purity>90%
AA SequenceMAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREI ELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGI DKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDG TGLEDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAV AKAGKPLVIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTV ISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVGQIRKQIEEATSDY DREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVDDALHATRAAVEEGVVAGGGVALV RVAAKLAGLTAQNEDQNVGIKVALRAMEAPLRQIVSNAGEEPSVVTNNVKAGEGNYGYNA ATEEYGNMIDFGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKGDAPDLXAAGMGG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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