Request QuoteCatalog Number: xP303247ESPSize: 0.2-1mg

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Recombinant 5,10-methylenetetrahydrofolate reductase (metF)

Recombinant 5,10-methylenetetrahydrofolate reductase (metF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP303247ESPYeast1mgQuote
EP303247ESPE. coli1mgQuote
BP303247ESPBaculovirus200ugQuote
MP303247ESPMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
UniProt IDP71319
Gene NamemetF
Protein Name5,10-methylenetetrahydrofolate reductase
Region Expressed1-298
Expression Tag6xHis
Purity>90%
AA SequenceMSFFHANQREALNQSLAELQGRINVSFEFFPPRTSDMEETLWSSIDRLSSLKPKFVSVTY GANSGERDRTHSIIKTIKERTGLEAAPHLTCIDASREQLREIAQDYWESGIRHIVALRGD LPQEGGKPDMYAADLVSLLKEVGDFDISVAAYPEVHPEAKSAQADLINLKHKIDAGANRA ITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQKFATMTNVRVPNWMTTMFD GLDNDPETRKMVGASIAMDMVKILSREGVKDFHFYTLNRAELSYAICHTLGVRPDVAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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