Request QuoteCatalog Number: xP527812DNVSize: 0.2-1mg

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Recombinant 5,10-methylenetetrahydrofolate reductase (metF)

Recombinant 5,10-methylenetetrahydrofolate reductase (metF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP527812DNVYeast1mgQuote
EP527812DNVE. coli1mgQuote
BP527812DNVBaculovirus200ugQuote
MP527812DNVMammalian Cell200ugQuote

Protein Information

SpeciesAquifex aeolicus (strain VF5)
UniProt IDO67422
Gene NamemetF; Locus:aq_1429
Protein Name5,10-methylenetetrahydrofolate reductase
Region Expressed1-296
Expression Tag6xHis
Purity>90%
AA SequenceMKIGDILRKGVFSISFEFFPPKTEEGERQLFETIRKLEKLNPTFVSVTYGAGGSTRDRTR NIVQKIHEETNLTVMAHLTCIAHTREELIDILQDYKNIGIENILALRGDVPRDKPDWRPP KGACKYAKELVELIRKEFGDWFSIGVASYPEGHPESPNLEWEVKYFKEKVEAGADFSITQ MFFVNDYYYRFVEMCKNAGIDISIIPGIMPITNFKQIRKFASLCGATIPQSLIEKLEKVE DKPEEVKKIGIEFAINQCLDLIEHGVPGLHFYTLNKSDATLKIYEAIKDKIPARST
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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