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Recombinant 23S rRNA (uracil (747) -C (5) ) -methyltransferase RlmC

Recombinant 23S rRNA (uracil (747) -C (5) ) -methyltransferase RlmC can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP502188VEZYeast1mgQuote
EP502188VEZE. coli1mgQuote
BP502188VEZBaculovirus200ugQuote
MP502188VEZMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain M66-2)
UniProt IDC3LWJ3
Gene NamerlmC; aka: rumB; Locus:VCM66_A0889
Protein Name23S rRNA (uracil (747) -C (5) ) -methyltransferase RlmC
Region Expressed1-375
Expression Tag6xHis
Purity>90%
AA SequenceMQCEFFIQKRCTSCHQCAQPYSQQVENKDQQLRELIAPAMDVQWLPPVTSADTAFRNKAK MVVLGAAHAPILGIEDAQGQPLSLVTCPLYPQPMQELLAYLENWIRIAGIPPYNKLKKKG ELKFILLTRSENSGQFMLRFVARSHAVLERIERNLPTLIAAFPTIEVVSVNIQPVHMARL EGEEEIFLTETQSLLEQFNDVPMVIRPKSFFQTNPQVAEQLYATARAWVREIAPTQMWDL FCGVGGFALHCAAPNTAVTGIEIEPEAIASAQRSAQMMGIDNLSFAALDSAKFSQSQMSA PELVLVNPPRRGLGSELTAQLEALAPQHILYSSCNPQTMVKDIAELASYQMSRVQWFDMF PHTDHAEVLTLLVRK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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