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Recombinant 23S rRNA (uracil (1939) -C (5) ) -methyltransferase RlmD

Recombinant 23S rRNA (uracil (1939) -C (5) ) -methyltransferase RlmD can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518983AZXYeast1mgQuote
EP518983AZXE. coli1mgQuote
BP518983AZXBaculovirus200ugQuote
MP518983AZXMammalian Cell200ugQuote

Protein Information

SpeciesAlteromonas macleodii (strain DSM 17117 / Deep ecotype)
UniProt IDF2GA29
Gene NamerlmD; Locus:MADE_1004040
Protein Name23S rRNA (uracil (1939) -C (5) ) -methyltransferase RlmD
Region Expressed1-482
Expression Tag6xHis
Purity>90%
AA SequenceMANLFKQSRAKQKNKTTPSQTQTSTKGSARANAQVAGLRFKKSKQSKEHNTAQDANNNAI TIQELDWMGQGVARGATMYFVEGALPGETCDIEVVSSKKKVVSAKTISIQGPSELRQPPF CPVFEACGGCQLQHIDADAALAYRDNALKIMMERQLSIGSGVWQAPLVGPRPQYRRKARL AIDARNPDNIKLGFREANSNKVVNLDTCPILVNPLSTAIGPLRNALEGYDSARHIGHISL IAGDNRAQVVIKHTKALEDALIETVDAFAKALGLDVVLENKQGRLRSVGEAQGLIMHTVD GCSISPSANDFIQINKVVNEKMINQALVWLDPRPNERIADWFSGLGNFTLPIAKKGALVQ AIEGVAEMVLRAKDNAQQQGIENVEWLHLDLANKANVEASLQQGFDKVLLDPSREGALTV CHALVKALPKTIVYVSCNPSTFSRDAKVLINGGYEMEKAGVAEMFPFTHHMEMMALFTQR QQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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