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Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)

Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP766372CKAYeast1mgQuote
EP766372CKAE. coli1mgQuote
BP766372CKABaculovirus200ugQuote
MP766372CKAMammalian Cell200ugQuote

Protein Information

SpeciesChromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
UniProt IDQ7P0W5
Gene NamedapD; Locus:CV_0450
Protein Name2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Region Expressed1-273
Expression Tag6xHis
Purity>90%
AA SequenceMHPIQSLIEQAFENRAEITPATVSPELKAAIEQVITELDNGHLRVAEKNGAEWVVNQWVK KAVLLSFRIRDNAVQDDGVNRYFDKVDTKFADWSQARFQEAGFRVVPGAVARKGSFIAKN TVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIED NCFIGARSEIVEGVIVGEGSVISMGVYIGQSTKIYDRETGEVMYGRVPPGSVVVSGNLPS KDGSHSLYCAVIVKKVDAQTRSKTSINELLRGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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