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Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)

Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388695STOYeast1mgQuote
EP388695STOE. coli1mgQuote
BP388695STOBaculovirus200ugQuote
MP388695STOMammalian Cell200ugQuote

Protein Information

SpeciesShewanella loihica (strain ATCC BAA-1088 / PV-4)
UniProt IDA3QJM4
Gene NamegpmI; Locus:Shew_3806
Protein Name2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMTTRKRPLALLILDGWGYRENTHMNAVYHANTPVLDRLNKEYPNSLISGSGLDVGLPDGQ MGNSEVGHINIGSGRVVYQELTRIGKSIEDGEFDSNPAFVDAVDKAIAADGAVHIMGLLS PGGVHSHESHIQAMCKMAVARGAKQVYLHAFLDGRDTPPRSAKGSLQAFNELFAELGTGR IASLIGRYYAMDRDNRWDRVSQAYELITQGKALHLYQDAVEALDAAYGRDENDEFVASSA ITDADGQVATLNDNDALIFMNFRADRARQITRSFVDPGFDGFERAVTPKINFVMLTEYAA DIKAPVAYPSVDLVNTLGEVLQNNGKTQLRISETEKYAHVTFFFNGGKEEPFEGEDRILI QSPKVATYDLQPEMSSTELTDKLVEAIESTQYDVIICNYPNGDMVGHTGNFDAAVKACEA VDACIGRVVEALAKVDGECLITADHGNAEQMTDEQTGQAHTAHTSELVPLIYVGRKGEIA PNGRLSDLAPTMLTLMGQTVPSEMTGHSIITLSE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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