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Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)

Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP766009WBQYeast1mgQuote
EP766009WBQE. coli1mgQuote
BP766009WBQBaculovirus200ugQuote
MP766009WBQMammalian Cell200ugQuote

Protein Information

SpeciesWolinella succinogenes
UniProt IDQ7M7W9
Gene NamegpmI; aka: pgm; Locus:WS2039
Protein Name2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Region Expressed1-492
Expression Tag6xHis
Purity>90%
AA SequenceMAQKTLLIITDGIGHKPSSPHNAFTQAKKPTYDSLFASAPHALLSTHGLSVGLPEGQMGN SEVGHMCIGAGRILYQDLVKISLALKDGSLEKNPTLLGFSSRAKRIHLAGLASDGGVHSH MEHLLGLAQIFSRLGHEVLLHLITDGRDVSPTSSKEFIAQALALESDLIKIATISGRYYA MDRDKRWDRIKRAFGVIARGDSPTSLTPQEYIQAQYQAGITDEFIEPAALGGYQGFEPSD GFVFANFRSDRAREIVSALGGASFEGFDRGAYQPPKTLAMTSYDDSFPFPILFPKEKVEP TLAQVISEAGLSQLHVAETEKYAHVTFFFNGGIETPWENESRVLIPSPSVATYDLKPEMS APEVAEATLSGMRQGYDFIVVNFANGDMVGHTGNTEAAISAVESVDRELGRLLEEAKKSG YAVILTSDHGNCEEMRDERGSMLTNHTVGEVWCFILAQGVTEIREGGLNQVAPTVLKIMG LPTPKEMDSPLF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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