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Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)

Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP516682BQXYeast1mgQuote
EP516682BQXE. coli1mgQuote
BP516682BQXBaculovirus200ugQuote
MP516682BQXMammalian Cell200ugQuote

Protein Information

SpeciesBacillus megaterium (strain DSM 319)
UniProt IDD5DNA8
Gene NamegpmI; aka: pgm; Locus:BMD_5035
Protein Name2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Region Expressed1-510
Expression Tag6xHis
Purity>90%
AA SequenceMSKKPVALIILDGFALRDEDKGNAVTHAKKPNFDRFWNEYPHATLQASGEAVGLPEGQMG NSEVGHLNIGAGRIVYQSLTRVNVAIREGEFEQNETLLAAVKHAKEKGTNLHLFGLLSDG GVHSHIEHLYALLRLAKSEGLEKVYIHGFLDGRDVAPQSAETYLKELNEKIEEYGVGEIA TLSGRYYSMDRDKRWERVEKSYRAMVYGEGPSYTSAEECVKDSYENGIYDEFVLPSVITK EDGSPVATIQDEDAVIFYNFRPDRAIQISNTFANEDFRSFDRGEKHPKNLHFVCLTHFSE TVDGYVAFKPNNLDNTLGEVLSQNNLKQLRIAETEKYPHVTFFMSGGREAEFPGETRILI DSPKVATYDLKPEMSAYEVTDALLAEIEGDKQDAILLNFANPDMVGHSGMLEPTVKAIET VDECLGKIVDAILAKGGTAIITADHGNADEVITLEGNPMTAHTTNPVPVIVTKQGLELRE DGILGDLAPTMLTLLDVAQPKEMTGKTLIK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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