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Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)

Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP463163DQYYeast1mgQuote
EP463163DQYE. coli1mgQuote
BP463163DQYBaculovirus200ugQuote
MP463163DQYMammalian Cell200ugQuote

Protein Information

SpeciesCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
UniProt IDB3PGV8
Gene NamegpmI; Locus:CJA_3553
Protein Name
Region Expressed1-516
Expression Tag6xHis
Purity>90%
AA SequenceMSAKKPVVLLILDGYGYSERTKYNAVYAAQTPVYDNLWKTCPNTLIDTSGMAVGLPAGQM GNSEVGHTTIGAGRVVYQSFTRINKSISDGDFFTNPEYVKAIDSAIANDRAVHILGLLSE GGVHSHQDHLYAMINMAVERGAKQVYLHAFLDGRDTPPRSAEASLQKAQDVFAKLGTGRV ASIVGRYFALDRDNRWDRVKTAYDVMVTGEAEFDALTAVDGLKAAYERGENDEFVKATVI CGEDEEVATINDGDSVIFMNFRPDRAREITRALIDENFTGFDRGETHPAIAHFVQTTEYA SDIKAPIAFPPEDLSNSFGEYIAGLGKTQLRIAETEKYAHVTFFFNGGNEVVYPGEDRIL VPSPKVATYDLQPEMSAPEVTDKLVEAIESGKYDAIICNYANCDMVGHSGLFDAAVKAVE AVDVALGRVLAAVKKVDGEALITADHGNVEEMFDEETGQPHTQHSTLPVPFIFVSSRKGK LASGGSLADVAPTILALMDLPQPKEMTGRNLITLEG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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