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Recombinant 14-3-3-like protein GF14 phi (GRF4)

Recombinant 14-3-3-like protein GF14 phi (GRF4) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP338421DOAYeast1mgQuote
EP338421DOAE. coli1mgQuote
BP338421DOABaculovirus200ugQuote
MP338421DOAMammalian Cell200ugQuote

Protein Information

SpeciesArabidopsis thaliana (Mouse-ear cress)
UniProt IDP46077
Gene NameGRF4; Locus:At1g35160; ORFs:T32G9.30
Protein Name14-3-3-like protein GF14 phi
Region Expressed1-267
Expression Tag6xHis
Purity>90%
AA SequenceMAAPPASSSAREEFVYLAKLAEQAERYEEMVEFMEKVAEAVDKDELTVEERNLLSVAYKN VIGARRASWRIISSIEQKEESRGNDDHVTTIRDYRSKIESELSKICDGILKLLDTRLVPA SANGDSKVFYLKMKGDYHRYLAEFKTGQERKDAAEHTLTAYKAAQDIANAELAPTHPIRL GLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLW TSDMQDESPEEIKEAAAPKPAEEQKEI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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