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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388142PZEYeast1mgQuote
EP388142PZEE. coli1mgQuote
BP388142PZEBaculovirus200ugQuote
MP388142PZEMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9301)
UniProt IDA3PBV8
Gene NameglgB; Locus:P9301_06101
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-754
Expression Tag6xHis
Purity>90%
AA SequenceMIETIQADWIKSEAINLENCCNDNPLKILGPHFYEEQWVIRVWVPEADEVKVNFKDKTYK AESINHKWLFEAILPENPNSNYEINISRGGITHTQHDPWSYREEWMGEVDRHLFAEGNHH HIWEKMGAHLIEDKNQKGVMFCIWAPNAKSISIIGDINSWDGRHNPMQKRLGGIWELFMP MMKEGDKYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIVSKLKNFNWSDSSWISNRDSS SQINKPISVYEMHLGSWLHESTDNKYLDDNGEPRDPVPAADLKPGTRLLTYPELTKKLIP YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGTPNEFREFVNKCHEEGIGVIL DWVPGHFPKDKHGLAFFDGCHLYEHGDSRIGEHKEWGTLIFNYSRNEVRNFLVANLVYWF EEFHIDGIRVDAVASMLYRDYLRPDGEWIPNENGGNENIEAVKFLQQANHVLFQHFPGAL SIAEESTTWPMVTKPTDMGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYN YTENFMLALSHDEVVHGKSHLLHKMPGDDWKKYANTRALLTYMWTHPGKKTIFMGMEFGQ RQEWNVWDDLQWELLEFEPHRGIRNLIDDLNKLYKNEPALWKNDFDPYGFQWIDCNDKSN SVISFMRRESDTNEWLVIVANFTPNTHGSYKIGVPVEGFYKEIFNSDGSRYGGSNKGNMG GKDTINYNIHDYQNALELALPPLSVSIFKHQSEK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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