Request QuoteCatalog Number: xP370736MOPSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP370736MOPYeast1mgQuote
EP370736MOPE. coli1mgQuote
BP370736MOPBaculovirus200ugQuote
MP370736MOPMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium ulcerans (strain Agy99)
UniProt IDA0PUI6
Gene NameglgB; Locus:MUL_3936
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-731
Expression Tag6xHis
Purity>90%
AA SequenceMSRTDQLAGTHLAPEPNDLALLVAGSHHNPHGVLGAHEYGDHIVIRAFRPHAAEVIAIVG DDRFPMQHIESGLFAVALPFVDLIDYRLQVSYENSEPFTVADAYRFLPTLGEVDLHLFAE GRHERLWEALGAHPRSFTTADGVVHGVSFAVWAPNAEGVSLIGDFNGWSGSEAPMRVLGS SGVWELFWPDFPADGLYKFRVHGADGVVSERADPFAFGTEVPPQTASRVTVSDYSWGDGD WMTERAQRNPVFEPMSTYEVHLGSWRPGLSYRQLATELTDYVVAHKFTHVELLPVAEHPF AGSWGYQVTSYYAPTSRFGTPDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGT PLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWIEQFHIDGLRVDAVASMLYLD YSRPQDGWTPNIYEGRENLEAVQFLQEMNATAHKAAPGIVTIAEESTSWPGVTRPTSIGG LGFSMKWNMGWMHDTLDYVSRDPIYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGT LWGRMPGSNHTKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGF SNGVLRLVRDINEIYCDHCALWSQDTTPEGYSWIDANDSANNVLSFLRHGSDGSVMACIF NFAGSEHSDYRLGLPIAGRWREVLNTDATIYNGSGVGNFGGVDATAEPWHGRPASAVLVL PPSSALWLEPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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