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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP873944CIUYeast1mgQuote
EP873944CIUE. coli1mgQuote
BP873944CIUBaculovirus200ugQuote
MP873944CIUMammalian Cell200ugQuote

Protein Information

SpeciesChlamydia muridarum (strain MoPn / Nigg)
UniProt IDQ9PL51
Gene NameglgB; Locus:TC_0257
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-737
Expression Tag6xHis
Purity>90%
AA SequenceMDPFFLNTQHVELLVSGKQSSPQDLLGIVSESLNQDRIVLFRPGAKTVFVELQGKIQQAE SHHSGIFSLPVTKGISPQDYRVYHQNGLLAHDPYAFPLLWGEIDSFLFHEGTHQHIYDRM GAIPCEIGGVPGVRFVIWAPHAQRVSVVGDFNCWHGLVNPLHKVSDQGVWELFVPGLTAG SCYKWEIVTASGQVLIKSDPYGKFFGSPPQSVSVVVDDRYEWGDKEWLDERSKKAEGPMN IYEVHVGSWRWQEEQPLNYRELADQLSLYCKQMHYTHVELLPVTEHPLNESWGYQTTGYY APTSRYGSFEDLQYFIDTMHQHGIGVILDWVPGHFPVDSFAMSNFDGTPLYEYTRNPSPL HPHWHTYTFDYAKPEVCNFLLGSALFWIDKMHIDGIRVDAVSSMLYLDYGRNPGEWIPNR YGGRENLDAVRFLQQFNTIIHEKYPGVLTFAEESTTFPKITVSVEEGGLGFDYKWNMGWM HDTLHYFEKDFPYRPYHQSDLTFPQWYAFSERFLLPFSHDEVVHGKRSLIGKMPGDAWRQ FAQLRLLLGYQICQPGKKLLFMGGEFGQGREWSPNRELDWDLLDIHYHQGVHLCSQKLNS LYVNSPQLWKGDHLPQSFRWIDFSDTRNGVVAYLRFASDEDKEALLCVHHFGVNHFLHYI LPMLPMKSCSLLMNTDDLAFGGSGKGFRNPKMLTPEMVRKEKTSSELAPLDDDGSVAWGL DIEMPPSATLIFSVTLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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