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Recombinant 1,4-alpha-glucan branching enzyme TK1436 (TK1436)

Recombinant 1,4-alpha-glucan branching enzyme TK1436 (TK1436) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP689290FHYYeast1mgQuote
EP689290FHYE. coli1mgQuote
BP689290FHYBaculovirus200ugQuote
MP689290FHYMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1) )
UniProt IDQ5JDJ7
Gene NameLocus:TK1436
Protein Name1,4-alpha-glucan branching enzyme TK1436
Region Expressed1-675
Expression Tag6xHis
Purity>90%
AA SequenceMKGYLTFVLHTHIPYVRKHGKWPFGEEWVFEAISETYIPLLMEFERLRDSGVKFGIVINV TPVLAEQLTDEYMKKAFEEYMERKLKAMEEDLKSGKYDEKAVSYMLNYFRKVYDYWKAIN GDIIGKLRELQDQGYVEVITSAATHGYLPLLGRDEAIRAQIANGVATYEKHFGMKPKGIW LPECAYRPAGEWELPGGRKVKRQGIEKFLEEFGLRYFFVESRLIDEGPASNVYGEVLIAD TEKTTLRPYWIKGSNVAVFARNRETGHQVWSAHYGYPGDFWYREFHKKAPKSGGQYWRIT SKEVGLGEKEFYDPDKAMERVEEHARHFVSLVERLLREHEEKFGEKGIIVAPYDTELFGH WWFEGVKWLGRVLELLYQRGVETPTLSRFLEEYSGEKHEIELPEGSWGANSDHSTWWNEE TEWTWPHIYRAEDRMVAIVSRFRGRDELTNRVIEQLARELLILEASDWQFLITTGQAKEY AKRRVLIHSRDFHRLANELVRYVKIGEFDVKLLEELEERDNPFRPVVVGPYVSENPPELE EYVEPPEVPPEKEETEEKPKVLTEKATSLALAVKKVKPVKEETREVKKKAVEASKRGKRK SSKSKRLPRKVSKKAPSKGPSDLLSIKGIGPKTFQKLKRAGVETIEDLKNANIEDLARKT GISTKRLKKFIAQVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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