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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP723125Yeast1mgQuote
EP723125E. coli1mgQuote
BP723125Baculovirus200ugQuote
MP723125Mammalian Cell200ugQuote

Protein Information

SpeciesNocardia farcinica (strain IFM 10152)
UniProt IDQ5Z0W8
Gene NameglgB; Locus:NFA_10780
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-744
Expression Tag6xHis
Purity>90%
AA SequenceMSGPEDPADRRHGEVPAPRRDIPRPLVERRDLMLLAAGTHRDPHTVLGAHPHPDGTVVRV LRPHAAKVSVRVGGVDHPLDSVGYGVFAAVLPHPELMDYRIVTEYPGGPTVIAADGFRFL PTLGELDLHLIGEGRHEHLWHVLGAHPRRYTTLDGPVSGTSFAVWAPNARGVTVIGDFDG WSGNTAPMRALGTSGVWEVFVPDVGSGTKYKYRVHGADGRVVDHADPLAFATELPPATAS VVTGSDYTWQDAQWLERRRSTDPTRSPMSVYEVHLGSWRPGLGYRELAEQLADYVRAAGY THVELLPVAEHPFGGSWGYQVTSYYAPTARFGSPDDFRAFVDHLHGAGIGVLLDWVPAHF PRDEWALARFDGTPLYEHPDPRRGEQLDWGTYVFDFGRNEVRNFLVANARFWIEEFHIDG LRVDAVASMLYLDYSRGENWEPNIHGGRENLEAVAFLRELNDVVHRAHPGVVTIAEESTT WPGVTRGTEVGGLGFTMKWNMGWMHDTLGFLARDPIHRSWHHNEITFSLVYAWSENYVLP ISHDEVVHGKGTLWTRMPGDDFAKAAGVRALLAYMWAHPGKQLLFMGQDFGQFREWSHDR GLDWGELDNPLHRGIATAVRDLNRVYRATPALWSQDTTPGGYAWIEADDRDRNVLAFLRY GSDGSTVACVFNFSGALHGEYRVGLPIAGEWTEILNTDAADYGGSGVGNYGVVRTEEIPW HGRPVSATVALAPNSAVWLAAPRA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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