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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP666730Yeast1mgQuote
EP666730E. coli1mgQuote
BP666730Baculovirus200ugQuote
MP666730Mammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain CC9902)
UniProt IDQ3AYE2
Gene NameglgB; Locus:Syncc9902_0919
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-764
Expression Tag6xHis
Purity>90%
AA SequenceMGGAAVLDWMVQDGVRLENCRHDHPLAVLGPQPDDQGWTVRVWMPEAQKVTLLLGSQEIV TSTPNHPWIFEASTPSDPGSNYKLRVERGGITTEQHDPWAFRHEWMGEMDRHLFAEGNHH HIWQRMGAHLTQIDGISGVMFCLWAPNALTASVLGDLNSWDGRHHPMQKRLGGIWELFVP GLDAGTLYKYEIRSQEGHCYQKADPYGFQHEVRPDNSSVVARLEGYSWSDSSWMQDRDSR NALDQPISVYEMHIGSWIHASADEPWIQPDGQPRAPVPAADMKPGARLLTYAELSDRLIP YVKERGFTHIELMPITEHPFDGSWGYQVTGWYAPTSRYGTPDEFRAFVDRCHAEGIGVII DWVPGHFPKDAHGLAFFDGTHLYEHGDPRIGEHKEWGTLIFNYSRNEVRNFLVANLVFWF EQFHIDGIRVDAVASMLYRDYLRPDGEWLPNENGGRENTEAVRFLQQANHVLFQHYPGAL SIAEESTTWPMVTQPTDMGGLGFNLKWNMGWMHDMLDYFELDPWFRQFHQNNITFSIWYT YTENFMLALSHDEVVHGKSHLLHKMPGDDWQKYANTRALLAYMWTHPGKKTIFMGMEFGQ RAEWNVWGDLEWDLLNYEPHKGIQRLVDDLNVLYKAQPALWRDDFDQFGFQWIDCNDNRH SVISFMRRDSASGTWLVVVANFTPQSHANYRVGVPLEGFYEEIFNTDAAKYGGSNLGNMG GKPTDACGIHGYEHSLDLCLPPLSLVVFQHDPKRTLIESSAPSD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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