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Recombinant 1,4-alpha-glucan branching enzyme GlgB 1 (glgB1)

Recombinant 1,4-alpha-glucan branching enzyme GlgB 1 (glgB1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP685428FOBYeast1mgQuote
EP685428FOBE. coli1mgQuote
BP685428FOBBaculovirus200ugQuote
MP685428FOBMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces coelicolor (strain ATCC BAA-471 / A3 (2) / M145)
UniProt IDQ59833
Gene NameglgB1; aka: glgBI; Locus:SCO5440; ORFs:SC6A11.16c
Protein Name1,4-alpha-glucan branching enzyme GlgB 1
Region Expressed1-774
Expression Tag6xHis
Purity>90%
AA SequenceMTPRPSSSGPDPRKTTGKKPAGKTPTGKKPAKAAKKKAPRRTTASANASATTSVSGAEVA VSPAPDAADRERLLAGTHHDPHAVLGAHRVPGGVAFRVFRPYALAVTVLSGELRVGLHDD GDGFFSGLVPLKDVPAHRLLVAYEGTEQEVEDPYRFLPTLGELDLHLLGEGRHEQLWRAL GAHPTTHEGVAGTRFAVWAPNARGVRVAGGFNFWDGTGHPMRSLGSTGVWELFLPGVGAG ELYKFEITRPDGSRTFRADPLARRTEVPPATSSVVHASDYTWGDEEWLAHRADAPAHEAP MSVYEVHLPSWRPGLTYRQLAEQLPAYVADLGFTHVELMPVAEHPFGGSWGYQVTGFYAP TARLGDPDDFKYLVDRLHRAGIGVLMDWVPAHFPRDDWALAEFDGRPLYEHSDPLRAAHP DWGTLEFDFGRREVRNFLVANAVYWCEEFHIDGLRVDAVASMLYLDYSREPGEWEPNEHG GRENLDAVAFLQEMNATLYRRVPGVVTVAEESTAWDGVTRATHHEGPSGFGGLGFGLKWN MGWMHDSLDYMSHEPVHRKHHHGEMTFSMVYAYSENYVLPISHDEVVHGKRSLVSKMPGD WWQQRANERAYLGFMWAHPGKQLLFMGQEFAQGAEWSEAHGPDWWLLDPEYGASADHRGV RDLVRDLNTVYRATPALWRRDTHPSGFSWVVGDAAEDNVLAFLRLDADGTPLLAVSNFAP VVRSGYRLGVPDEVPAWHEVLNTDAARYGGGDVVNPDPVKPEPQGGTAARRASG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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