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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP603271Yeast1mgQuote
EP603271E. coli1mgQuote
BP603271Baculovirus200ugQuote
MP603271Mammalian Cell200ugQuote

Protein Information

SpeciesNitrosomonas eutropha (strain C91)
UniProt IDQ0AGJ0
Gene NameglgB; Locus:Neut_1291
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-732
Expression Tag6xHis
Purity>90%
AA SequenceMKSLPFPSPSRDLAGDSAQSLLLAARLHAPGSYLGIHSVPGGELVRVFQPYMSRVWLQTS SGFQVMECTHDAGIFEWKGEAVTRPYLLRLEHAETGVIEERYDPYAFPVQISGHDLYLFN EGRLLQAYHMLGTHQVKNQGVTGTRFAVWAPNAERVSVVGDFNHWDGRVCPMMARDHSGV WELFIPDLPGGTLYKYEIRNHSTGEILLKTDPYATRYELRPNNAALTPVESRYEWQDNDW MVQRAGWDWLHAPVNIYELHVGSWKRHPDGSFYSYQELADHLIPYLQEMGYSHVELLPIS EHPLDESWGYQVTGYFAATSRYGNPEAFMYFVDKCHQAGIGVILDWVPAHFPQDSFSLAR FDGTALYEHEDPRLGYHQDWGTYIFNYGRSEVKSFLLSSAHYWLSVFHIDGLRVDAVASM LYLDYSRKEGEWLPNRFGGRENLDAIDFLRELNTMVHGEFSGALTFAEESTSWPAVSRPT YLGGLGFSMKWNMGWMNDTLNYMQLDPIHRHYHHNELTFNQLYAYTENFILPLSHDEVVH GKKSLLDKMSGDVWQMFANLRLLFTYQMTCPGKKINFMGNEFAQGREWRVNHELDWYLLE RDPHRGIQMLLRNLNHLYLDTPALHELDFFTEGFSWIDCHDSEQSVISYQRRARDGSFML VILNFTPVPRTGYRVGVPESRTYQEVFNSDSTYYGGSNIGNPGDIVPTGQQWSGQVDSII ITLPPLAGIILI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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