Request QuoteCatalog Number: xP638233Size: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP638233Yeast1mgQuote
EP638233E. coli1mgQuote
BP638233Baculovirus200ugQuote
MP638233Mammalian Cell200ugQuote

Protein Information

SpeciesNitrobacter hamburgensis (strain X14 / DSM 10229)
UniProt IDQ1QNB1
Gene NameglgB; Locus:Nham_1464
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-716
Expression Tag6xHis
Purity>90%
AA SequenceMIELSSEARAIVEGRHSDPFHYLGRHRENDRQVVRAYLPEASDVRVVDEHGGETTLTRIH DAGLFLGDLPNGAKRYRLRARFGADTVELEDPYRFPPILSDFDLYLLGEGTDQRLYDKLG AHPITMDGVGGTGFVVLAPNARAVAVVGDFNCWDRRRHPMRVRGNGYWELFIPGAQTGDR YKFAITDKSGSLLPLKSDPMAFAAEVRPNTASIVVDEAALPCPTPAAATINARRAPISIY EVHLGSWRRGDDNRWLSYRELADTLPRYASSLGFTHIELMPVNEHPFDGSWGYQPTGLYA PTSRFGSPDDFAHLVDACHREGLGVLLDWVPGHFPDDPHGLGHFDGTAFYEHANPLQGRH LDWDTLIYNYGRTEVVNFLVANALFWLDRYGLDGLRVDAVASMLYLDYSRAEGGWIPNKH GGRENLEAIAFLRRFNREVFAKFPHATTIAEESTAWPQVSRPIEFGGLGFGYKWNMGWMH DTLDYIGKDPIYRKHHHGQILFGLHYAFSENFILPLSHDEVVHEKRSILGRMPGDDWQRF ANLRAYYAFMFGHPGKKLMFMGAEFGQEREWNHDHALDWHLLDQARHQGVQAVVRDLNAL YRDVPALHELDCDPAGFEWIMANDADRSVFAWMRKGESGRARCLVIANFTPTVHRSYRVR VPFAGKWREALNTDSAHYGGSNVGNNGVVETLDGVIPELSLTLPPLAVIFLVPERA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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