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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP611115Yeast1mgQuote
EP611115E. coli1mgQuote
BP611115Baculovirus200ugQuote
MP611115Mammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt) )
UniProt IDQ0I3H7
Gene NameglgB; Locus:HS_0889
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-807
Expression Tag6xHis
Purity>90%
AA SequenceMNKFVSQSTIDTFFDGKHADPFSVLGMHETSNGIEVRVLLPDAEKVFVLSKETKNVLCEL SRVDERGFFAGVVPNTHSFFAYQLEVYWGNEAQVIEDPYAFHPMINELDNWLLAEGSHKR PYEILGAHFTECDNVAGVNFRLWAPNAKRVSVVGDFNYWDGRRHPMRFHQSSGIWELFLP KVSLGQLYKFELLDCHNQLRLKADPYAFSSQLRPDTASQIGALPEIVSMTEKRRKANQAD QPISIYEVHLGSWRRNLENNFWLDYDQIAEELIPYVKDMGFTHIELLPLSEFPFDGSWGY QPIGLYSPTSRFGTAEGFKRLVNKAHKAGINVILDWVPGHFPSDTHGLVAFDGTALYEHA DPKEGYHQDWNTLIYNYGRHEVKNYLASNALYWMERFGLDGIRVDAVASMIYRDYSREDG QWIPNQYGGRENLEAIEFLKQTNHLLGTEIPGVVSIAEESTSFAGVTHPPYEGGLGFHFK WNMGWMNDTLSYMKKDPIYRQHHHSQMTFGMVYQYSENFILPLSHDEVVHGKCSLLGKMP GDAWQKFANLRAYYGYMWGYPGKKLLFMGNEFAQGREWNYQESLDWYLLDEFHGGGWHKA VQDYVRDLNHIYQKNAPLFELDTDPQGFEWLVVDDYQNSVFVFERRSKKDERIIVISNFT PVLRQNYRFGVNIAGEYMEILNSDAQQYMGSNSTNTSKIVTEDIESHNKAQSISIDIPPL ATVYLKLHKVKKVRKMRKTSKVEDTAVKTEKKIAKGKTTRTKKTVADTVSEATEVKPKKT VTKRAVVKKVKNAEVIAESTSVENNVS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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