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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP633540Yeast1mgQuote
EP633540E. coli1mgQuote
BP633540Baculovirus200ugQuote
MP633540Mammalian Cell200ugQuote

Protein Information

SpeciesLactobacillus salivarius (strain UCC118)
UniProt IDQ1WSM8
Gene NameglgB; Locus:LSL_1294
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-649
Expression Tag6xHis
Purity>90%
AA SequenceMAVNTEERHGQESLENHKHMFNQGVNYELYNFLGVHKISRDDESGYVFRVWAPHAKQVWV CGDFNNWDKSHPMILDEKSGIWSIEVKESRENQYYKYLVKQANGREIMKTDPMAQVYEKR PETAAVVKELEPFKWNDGLWRGRQKRMNHFNNPINIYEVHAPSWKEHEDGSLYTFKDLTA ELIPYVKKMGYTHIEFMPLMEHPLPASWGYQLTGYFALCSSYGTPDEFKDFVNECHQNNI GVLVDWVPGHFSINDDALAYYDGTPTYEYEDPDRAKNIGWGALNFDLGKPEVQSFLLSSA FYWLNEFHLDGMRVDAVSNMIYLDYDEGPWKPNKYGDTRNLEGYAFLQKFNKVVKFAHPE SLIIAEESSSGTQISGTIESGAIGFDYKWNMGWMNDVLEFFEMDPYFRKDNLNLVTFSFM YWQAENFVLPLSHDEVVHGKKSLMHKMWGDRYRQFAQLRTLYTFMMMHPGKKLLFMSGEW GQFLEWKFDHGLEWVDLQDEMNAKMQHFTSVLNHFYKEERPLWELGQQDATLEVIDADNH NDTVLSFIRHGKRKKDFLIVILNFTPVERRNFRIGVPYEGTYTEILNTEMKEFGGTWVEH NADSVSEEVNFKDYEHSITTTVPALGAIILKPKDIKVTRRSSKKHSHKK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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