Request QuoteCatalog Number: xP324586FPYSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP324586FPYYeast1mgQuote
EP324586FPYE. coli1mgQuote
BP324586FPYBaculovirus200ugQuote
MP324586FPYMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
UniProt IDP16954
Gene NameglgB; Locus:Synpcc7942_1085
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed2-774
Expression Tag6xHis
Purity>90%
AA SequenceTGTTPLPSSSLSVEQVNRIASNQEQNPFDILGPHPYEHEGQAGWVIRAYLPEAQEAAVIC PALRREFAMHPVHHPHFFETWVPEETLEIYQLRITEGERERIIYDPYAFRSPLLTDYDIH LFAEGNHHRIYEKLGAHPCELENVAGVNFAVWAPSARNVSILGDFNSWDGRKHQMARRSN GIWELFIPELTVGAAYKYEIKNYDGHIYEKSDPYGFQQEVRPKTASIVADLDRYTWGDAD WLERRRHQEPLRQPISVYEVHLGSWMHASSDAIATDAQGKPLPPVPVADLKPGARFLTYR ELADRLIPYVLDLGYSHIELLPIAEHPFDGSWGYQVTGYYAATSRYGSPEDFMYFVDRCH QNGIGVILDWVPGHFPKDGHGLAFFDGTHLYEHADSRQGEHREWGTLVFNYGRHEVRNFL AANALFWFDKYHIDGIRVDAVASMLYLDYNRKEGEWIPNEYGGRENIEAADFLRQVNHLI FSYFPGALSIAEESTSWPMVSWPTYVGGLGFNLKWNMGWMHDMLDYFSMDPWFRQFHQNN VTFSIWYAFSENFMLALSHDEVVHGKSNLIGKMPGDEWQKFANLRCLLGYMFTHPGKKTL FMGMEFGQWAEWNVWGDLEWHLLQYEPHQGLKQFVKDLNHLYRNAPALYSEDCNQAGFEW IDCSDNRHSIVSFIRRAHESDRFLVVVCNFTPQPHAHYRIGVPVAGFYREIFNSDARSYG GSNMGNLGGKWTDEWSCHNRPYSLDLCLPPLTTLVLELASGPESLSEAANSPL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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