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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP708503CTLYeast1mgQuote
EP708503CTLE. coli1mgQuote
BP708503CTLBaculovirus200ugQuote
MP708503CTLMammalian Cell200ugQuote

Protein Information

SpeciesCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
UniProt IDQ5KD76
Gene NameADI1; Locus:CNH03570
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-187
Expression Tag6xHis
Purity>90%
AA SequenceMKAYIYDDKPGDQRLPHDTGIDIPEPTLAKLGVVYQRIPIDSEGAWESKIDEFAKERGYK NRDRITVTREGLGEAYEEKIKSFFDEHLHEDEEIRYILAGSGYFDIRGAEGVHEEQWIRI ALEAGDLIVLPAGIYHRFTVDSANTITAMRLFQDEPKWTPYSRKADGTDKLGSRDKYLET VRVGVTA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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