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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (Adi1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (Adi1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP707068RAYeast1mgQuote
EP707068RAE. coli1mgQuote
BP707068RABaculovirus200ugQuote
MP707068RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDQ562C9
Gene NameAdi1; aka: Alp1, Mtcbp1
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed2-179
Expression Tag6xHis
Purity>90%
AA SequenceVQAWYMDESTADPRMPHRAQPDRPVGLEQLRTLGVLYWKLDADKYENDPELEQIRKTRNY SWMDIITICKDSLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISMEK GDMITLPAGIYHRFTLDEKNYVKAMRLFVGEPVWTPYNRPADHFDARVQYVKFLEGTA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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