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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP506413AYZYeast1mgQuote
EP506413AYZE. coli1mgQuote
BP506413AYZBaculovirus200ugQuote
MP506413AYZMammalian Cell200ugQuote

Protein Information

SpeciesAjellomyces capsulata (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432) (Darling's disease fungus) (Histoplasma capsulatum)
UniProt IDC0NZU2
Gene NameADI1; ORFs:HCBG_08672
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-176
Expression Tag6xHis
Purity>90%
AA SequenceMKAYWYDNIPGDQRLPHDSGRTVDASYLATIGVLYRHCPAISAVDDLARDRGYKNRDEII ISPEKMGDVYEDKVRMFFNEHLHEDEEIRYIVDGEGFFDVRSKDDEWVRIRLGKGDLIVL PAGIYHRFTTDENNYVKAMRLFQDEPKWTPLNRAPELDANQHRKTYVESHISSSTA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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