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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515278MUEYeast1mgQuote
EP515278MUEE. coli1mgQuote
BP515278MUEBaculovirus200ugQuote
MP515278MUEMammalian Cell200ugQuote

Protein Information

SpeciesMonodelphis domestica (Gray short-tailed opossum)
UniProt IDF7FKV1
Gene NameADI1; aka: MTCBP1
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed2-179
Expression Tag6xHis
Purity>90%
AA SequenceVEAWYMDDSEEDQRRPHRLEPERPVSLAHLQQLGVFYWKLDADKYEDDPELEKIRKERKY CWMDIITICKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDKWIRIFMEK GDMITLPAGIYHRFTLDETNYVKAMRLFVGEPVWTAYNRPADHFEIRGQYLQFLAQKG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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