Request QuoteCatalog Number: xP515203FHISize: 0.2-1mg

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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515203FHIYeast1mgQuote
EP515203FHIE. coli1mgQuote
BP515203FHIBaculovirus200ugQuote
MP515203FHIMammalian Cell200ugQuote

Protein Information

SpeciesPuccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)
UniProt IDE3KY53
Gene NameADI1; ORFs:PGTG_15095
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-181
Expression Tag6xHis
Purity>90%
AA SequenceMRAYIYDEESQLSPQDEHESSQSVSKQELEKLGVLYWSIDSIDQVNSIAIQRDYKNRDQI ICSPQAMGDIYQQKLDTFFEEHLHEDEEIRWVVEGSGYFDVRDQTDQRWVRIKVEKGDLL VLPPGIFHRFTVDKDNYIKAMRLFKDEPKWVALNRSTESESNPYRKAYIEQVGKAMTMGA F
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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