$678.00Catalog Number: BXW1015-kaSize: 20 ug

pMEVHA-IKK2-K44A

Human IKK2 Xpress Clones

NF-kappa-B and REL proteins form the NF-kappa-B complex, which is inhibited by I-kappa-B proteins (including NFKBIA or NFKBIB). Binding of IκB will trap the NFκB complex in the cytoplasm. Phosphorylation on serine residues on the IkB protein by IKK1 or IKK2 will mark the protein for destruction via the ubiquitination pathway and allow the relocation of the NFkB complex to the nuclei for transcription functionalities. IKK1 or IKK2 may form homo- or hetero-dimer. The K44A or K44M mutation renders the protein kinase enzymatically inactive. The S177E / S181E mutant is potentially constitutively active while S177A / S181A mutant can no longer be activated by upstream kinases.

Includes and available separately

BXW1015-5:20 ug each
BXW1015-wt:20 ug
BXW1015-ka:20 ug
BXW1015-ee:20 ug
BXW1015-aa:20 ug
BXW1015-kmaa:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Kpn I
3' cloning site:Xba I
GenBank#:NM_001556
Mutations:K44A; S177E, S181E; S177A, S181A; K44M, S177A, S181A

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human IKK2 protein sequence

Human IKK2 nucleotide sequence

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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