$678.00Catalog Number: BXP1015-gvSize: 20 ug

pMEV2HA-NRAS-G12V

Human NRAS Xpress Clones

Ras proteins are members of small GTPase superfamily. They bind GDP/GTP and possess intrinsic GTPase activity. These proteins act as switches to turn on/off their downstream target proteins in response to upstream stimuli because they can alternate between an inactive form bound to GDP and an active form bound to GTP. Human NRAS cDNA was first cloned from human sarcoma cell lines (HT1080 and RD). Activating mutations of NRAS at codons 12, 13 or 62 were found in a variety of human tumors. S17N is a widely used dominant negative mutant.

Gene Name: Homo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog. Other Names: N-ras; NRAS1; neuroblastoma RAS viral (v-ras) oncogene homolog; v-ras neuroblastoma RAS viral oncogene homolog; Transforming protein N-Ras.

Includes and available separately

BXP1015-3:20 ug each
BXP1015-wt:20 ug
BXP1015-sn:20 ug
BXP1015-gv:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Eco RI
GenBank#:NM_002524
Mutations:S17N; G12V

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human NRAS protein sequence

MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY
RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC
VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL
PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV
REIRQYRMKK LNSSDDGTQG CMGLPCVVM

Human NRAS nucleotide sequence

ATGACTGAGT ACAAACTGGT GGTGGTTGGA GCAGGTGGTG TTGGGAAAAG
CGCACTGACA ATCCAGCTAA TCCAGAACCA CTTTGTAGAT GAATATGATC
CCACCATAGA GGATTCTTAC AGAAAACAAG TGGTTATAGA TGGTGAAACC
TGTTTGTTGG ACATACTGGA TACAGCTGGA CAAGAAGAGT ACAGTGCCAT
GAGAGACCAA TACATGAGGA CAGGCGAAGG CTTCCTCTGT GTATTTGCCA
TCAATAATAG CAAGTCATTT GCGGATATTA ACCTCTACAG GGAGCAGATT
AAGCGAGTAA AAGACTCGGA TGATGTACCT ATGGTGCTAG TGGGAAACAA
GTGTGATTTG CCAACAAGGA CAGTTGATAC AAAACAAGCC CACGAACTGG
CCAAGAGTTA CGGGATTCCA TTCATTGAAA CCTCAGCCAA GACCAGACAG
GGTGTTGAAG ATGCTTTTTA CACACTGGTA AGAGAAATAC GCCAGTACCG
AATGAAAAAA CTCAACAGCA GTGATGATGG GACTCAGGGT TGTATGGGAT
TGCCATGTGT GGTGATGTAA

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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