$678.00Catalog Number: BXP1025-snSize: 20 ug

pMEV2HA-KRAS2B-S17N

Human K-Ras 2B Xpress Clones

Ras proteins are members of small GTPase superfamily. They bind GDP/GTP and possess intrinsic GTPase activity. These proteins act as switches to turn on/off their downstream target proteins in response to upstream stimuli because they can alternate between an inactive form bound to GDP and an active form bound to GTP. Activating mutations of KRAS2, including G12V, have been implicated in various malignancies, including lung denocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. On the other hand, S17N is a widely used dominant negative mutant.

Alternative splicing of KRAS-2 gene leads to variants encoding two isoforms that differ in the C-terminal region. KRAS-2B variant terminates in exon 4b, encoding a unique C terminus and lacks exon 4a that is present in the longer transcript variant KRAS-2A.

Gene Name: Homo sapiens v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog (KRAS2), transcript variant B. Other Names: Transforming protein p21b, K-Ras 2B, Ki-Ras, c-K-ras

Includes and available separately

BXP1025-3:20 ug each
BXP1025-wt:20 ug
BXP1025-sn:20 ug
BXP1025-gv:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Xho I
GenBank#:NM_004985, P01118
Mutations:S17N; G12V

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human K-Ras 2B protein sequence

MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY
RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC
VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL
PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV
REIRKHKEKM SKDGKKKKKK SKTKCVIM

Human K-Ras 2B nucleotide sequence

ATGACTGAAT ATAAACTTGT GGTGGTTGGA GCTGGTGGCG TAGGCAAGAG
TGCCTTGACG ATACAGCTAA TTCAGAATCA TTTTGTGGAC GAATATGATC
CAACAATAGA GGATTCCTAC AGGAAGCAAG TAGTAATTGA TGGAGAAACC
TGTCTCTTGG ATATTCTCGA CACAGCAGGT CAAGAGGAGT ACAGTGCAAT
GAGGGACCAG TACATGAGGA CTGGGGAGGG CTTTCTTTGT GTATTTGCCA
TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT
AAAAGAGTTA AGGACTCTGA AGATGTACCT ATGGTCCTAG TAGGAAATAA
ATGTGATTTG CCTTCTAGAA CAGTAGACAC AAAACAGGCT CAGGACTTAG
CAAGAAGTTA TGGAATTCCT TTTATTGAAA CATCAGCAAA GACAAGACAG
GGTGTTGATG ATGCCTTCTA TACATTAGTT CGAGAAATTC GAAAACATAA
AGAAAAGATG AGCAAAGATG GTAAAAAGAA GAAAAAGAAG TCAAAGACAA
AGTGTGTAAT TATGTAA

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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