$954.00Catalog Number: BXP6050-wtSize: 20 ug

pKG-cJun (1-79)

GST-cJun Xpress Clones

Human c-Jun serves as phosphorylation target for JNK in vivo and in vitro. pKG-cJun(1-79) expresses a fusion protein of GST and human c-Jun amino acid residues 1-79 upon IPTG induction in an appropriate E. coli strain, such as DH5α, BL21, TG1, JM109 or XL1-Blue. pKG-cJun-M express the same fusion protein but with the JNK phosphorylation sites mutated, so it can be used as a negative control in in vitro kinase assays.

Includes and/or available separately

BXP6050-2:GST-cJun, set of 220 ug each
BXP6050-wt:pKG-cJun (1-79)20 ug
BXP6050-m:pKG-cJun-M (1-79)20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug high purity plasmids in 40ul TE buffer.
Antibiotic Selections:Amp
Cloning vector:pKG
Promoter:Lac
IPTG induction:Yes

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. If received in lyophilized form, the plasmid can be reconstituted in buffer of choice, preferably in 40ul of TE. It is recommended that you propagate the vectors in E. coli strains like DH5α, XL1-Blue, BL21, TG1 or JM109.

Induction for GST-cJun expression

  • Pick up a single colony and grow up in 10ml of LB plus 50-100 ug/ml ampicillin in a 37oC shaker for overnight
  • Inoculate 1L of LB+50 ug/ml of ampicillin in a 2L flask with the 10ml of overnight culture and grow in the 37oC shaking incubator
  • When the OD600 of the culture reaches 0.6, add 0.2 ml of IPTG stock solution (1.0 M) into the flask and continue shaking at 37oC for 4 hr
  • Harvest the cells by centrifugation at 5000 rpm for 5 min
  • The cell pellet can be used immediately for purification of GST-cJun protein. Or it can be frozen at -80oC for later use

Human c-Jun (aa1-79) nucleotide sequence

CAGACTGCAA AGATGGAAAC GACCTTCTAT GACGATGCCC
TCAACGCCTC GTTCCTCCCG TCCGAGAGCG GACCTTATGG
CTACAGTAAC CCCAAGATCC TGAAACAGAG CATGACCCTG
AACCTGGCCG ACCCAGTGGG GAGCCTGAAG CCGCACCTCC
GCGCCAAGAA CTCGGACCTC CTCACCTCGC CCGACGTGGG
GCTGCTCAAG CTGGCGTCGC CCGAGCTGGA GCGCCTGTGA
GAATTCATCG TGACT

pKG-cJun Vector Map

pkg_cjun.jpg

Quantity

$954.00

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