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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP489480EPVYeast1mgQuote
EP489480EPVE. coli1mgQuote
BP489480EPVBaculovirus200ugQuote
MP489480EPVMammalian Cell200ugQuote

Protein Information

SpeciesCyanothece sp. (strain PCC 7425 / ATCC 29141)
UniProt IDB8HY50
Gene NamehisA; Locus:Cyan7425_2496
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-257
Expression Tag6xHis
Purity>90%
AA SequenceMDIIPAIDLLGGRCVRLYQGDYNQAEVFDTDPVGMARRWLSQGATRLHLVDLDGAKTGEP VNQAAMAAIVQDLAIPVQVGGGVRSRQRVVELLDLGVDRVILGTVAIEQPQLVEALCAEF PGRILIGIDARDGKVATRGWLETSTVQATDLARTVEKAGAAGIIYTDIHRDGTLQGPNLE ALRQLASEVNLPIIASGGVSSIADLLNLFVLSTSGVTGVIIGKALYSGDITLTDAIRAVG QGRWQDVPPDLGFSTFA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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