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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP491738DKJYeast1mgQuote
EP491738DKJE. coli1mgQuote
BP491738DKJBaculovirus200ugQuote
MP491738DKJMammalian Cell200ugQuote

Protein Information

SpeciesDictyoglomus turgidum (strain Z-1310 / DSM 6724)
UniProt IDB8E2C7
Gene NamehisA; Locus:Dtur_1125
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAIDIYKSKVVRMETGKKEKIILEFDNPIDLAKYWEKKGAKALHLIDLQSAIDGID ENKNIIREIIKNVSIPVEVGGGYRDKEKIEEAINWGVWRVIVSSILEKDLGYLSDLFSKY KEKIIPSIDWYDGRVGIKGWQDFVEWRNIKDKLDLLRVKEVIFTDISRDGTLRGINVENI KTFLRLHDYDIWIAGGISSIEDIIKIKNIWKETERIKGVIIGRALLEGRINWEEAEDIIN AG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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