$90.00Catalog Number: PYT544-10ugSize: 10ug

LGALS1 Human

Galectin-1 Human Recombinant

LGALS1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 14.5 kDa.
The LGALS1 is purified by proprietary chromatographic techniques.

Accession

P09382

Amino acid sequence

ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.

Biological Activity

Human Galectin-1 is a chemoattractant for human blood monocytes. ED50 range is from 0.05-3.0 ug/ml corresponding to a specific activity of 0.3-20MIU/mg.

Formulation

The Galectin-1 protein was lyophilized from a concentrated (1mg/ml) containing 10mM sodium phosphate buffer, pH-7.5.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content

UV spectroscopy at 280 nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized LGALS1 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Galectin-1 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Galectin-1 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Galectin-1, GAL1, GAL-1, Lectin galactoside-binding soluble 1, Beta-galactoside- binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14 kDa lectin, HPL, HBL, Putative MAPK-activating protein PM12, GBP, DKFZp686E23103.

Quantity

$90.00

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