$148.20Catalog Number: PYT358-100ugSize: 100ug

IFN g Mouse

Interferon-Gamma Mouse Recombinant

Interferon-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15601 Dalton.
The IFN-gamma is purified by proprietary chromatographic techniques.

Accession

P01580

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Thr-Val.

Biological Activity

The specific activity as determined by the cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with EMC virus was < 0.1 ng/ml, corresponding to a specific activity of 10MU/mg.

Formulation

Lyophilized from a 0.2¦Ìm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IFN-gamma should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.

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Quantity

$148.20

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