$148.20Catalog Number: PYT331-10ugSize: 10ug

Flt3 Ligand Human

Flt3 Ligand Human Recombinant

Flt3-Ligand Human Recombinant produced in E.Coli is non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17605 Dalton. Flt3-Ligand is purified by proprietary chromatographic techniques.

Accession

P49771

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.

Biological Activity

The ED50 range=0.5-1.0 ng/ml corresponding to a specific activity of 1-2MUnits/mg, calculated by the dose-dependant stimulation of the proliferation of human OCMI-AML5 cells.

Formulation

The protein was lyophilized with no additives.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Flt3-Ligand should be stored at 4oC between 2-7 days and for future use below -18oC.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Quantity

$148.20

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