$90.00Catalog Number: PYT386-10ugSize: 10ug

FGF 2 Mouse

Fibroblast Growth Factor-Basic Mouse Recombinant

Fibroblast Growth Factor-basic Mouse Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16320 Dalton.
The FGF-2 is purified by proprietary chromatographic techniques.

Accession

P15655

Amino acid sequence

MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS.

Biological Activity

The activity as calculated by the dose-dependant proliferation of BALB/3T3 cells was found to be > 1ng/ml, corresponding to a specific activity of 1,000,000 units/mg.

Formulation

FGF-b was lyophilized from 5mM Na2PO4, pH7.5 and 50mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content

Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGF-basic should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.

Quantity

$90.00

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