$148.20Catalog Number: PYT325-10ugSize: 10ug

EPO a Fc Human

Erythropoietin-Alpha Fc-Chimera Human Recombinant

Erythropoietin-alpha Fc-Chimera Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a dimeric, glycosilated, polypeptide chain consisting of two mature human EPO molecules linked to the Fc portion of human IgG1. The Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 140 kDa in non-reducing SDS-PAGE.

Accession

P01588

Biological Activity

The ED50 as determined by the dose-dependant stimulation of human megakaryoblastic leukemia cells is less than 2.0 ng/ml, corresponding to a Specific Activity of 0.5MIU/mg.

Formulation

Each mg of lyophilized powder contains 1x PBS pH-7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Erythropoietin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Chinese Hamster Ovary Cells(CHO).

Stability

Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution EPO-alpha should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

EPO-α, EPO-alpha, Epoetin, EP, MGC138142.

Quantity

$148.20

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