Request QuoteCatalog Number: xP405242TKDSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP405242TKDYeast1mgQuote
EP405242TKDE. coli1mgQuote
BP405242TKDBaculovirus200ugQuote
MP405242TKDMammalian Cell200ugQuote

Protein Information

SpeciesThermosipho melanesiensis (strain BI429 / DSM 12029)
UniProt IDA6LJ47
Gene NamemiaB; Locus:Tmel_0071
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-430
Expression Tag6xHis
Purity>90%
AA SequenceMKKIHIKTYGCQMNENDSEVAKFYLEEEGYEITNNENDADIVILNTCVVRKKSEDKFYSH IGELKKQNKIIGIMGCGAEKEKEKLFKRGVKFVIGTRAIPLIPQAVERAINGKKSAIFED KMDEIDYKKILKRNSKHHAWITIIYGCNRFCTYCIVPYTRGREKSRKMDDIINEVENLAK SGIKEVTYLGQNVDAYGKDLNDGSSLAKLLNLTKDIEEIERIWFLTSYPTDFSLDIAHEV ANSSKITKNIHLPVQHGSNKILKKMNRRYTIEEYIELINDIRKIVPDASISSDIIVGFPD ETEEDFEKTVELVKNIKFERLNLAIYSPREGTIAWKYFEDNVPRIIKTKRMAYILNLQKE INKQLNENYLNKTVEIIVETKAKSGLYYGRDIRNKIIAFEGDKSLIGKKVLVKVKKTTAG PLYGDIIKIL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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