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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP406682PYBYeast1mgQuote
EP406682PYBE. coli1mgQuote
BP406682PYBBaculovirus200ugQuote
MP406682PYBMammalian Cell200ugQuote

Protein Information

SpeciesParabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152)
UniProt IDA6LEM6
Gene NamemiaB; Locus:BDI_2415
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMVNQENGVDLKSATQSDERKLFIETYGCQMNVADSEVVASIMKMDGYSMTENIEEADAIF VNTCSVRDNAEQKIYGRLQYFQSLKRKKKSLIVGVLGCMAERVKEDLIHVHHADLVVGPD SYLDLPNLVGAVEHGEKAINVELSTQETYKDVIPLKLPGVHISGFVSIMRGCNNFCTYCI VPYTRGRERSRDIESILNEIRDMHEKGFKEVTLLGQNVNSYSFEKEGETITFPILLDRVA KEVPDMRIRFTTSHPKDMSDDTLRVIAANDNICKFIHLPAQSGSSRILKVMNRKYTREWY LDRIAAIRRIVPDCAISTDLFCGFHSETEEDYQETLSLMREVGYDSAFLFKYSERPGTYA ASHLEDNVPEEEKVRRLQGMIDLQNKLSEESNLRDIGKTFEVLIEGFSKRSREQLFGRTS QNKVVIFDKKNYHVGQFIKVKIHKASSATLFGEPVEE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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