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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388680STOYeast1mgQuote
EP388680STOE. coli1mgQuote
BP388680STOBaculovirus200ugQuote
MP388680STOMammalian Cell200ugQuote

Protein Information

SpeciesShewanella loihica (strain ATCC BAA-1088 / PV-4)
UniProt IDA3QH41
Gene NamemiaB; Locus:Shew_2923
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLDEYQGYTLTEEAEEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDLIFGPQTLHRLPEMIEQIQQ GGKAVIDVSFPEIEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPVDD VILEIAQLAEQGVREVNLLGQNVNAYRGATHDDDICTFAELLRYVAAIDGIDRIRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDI QISSDFIVGFPGESKQDFADTMKLIEDVQFDHSFSFIYSARPGTPASDLPDDVSLDEKKE RLAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTENNRVVNFEADPKHIG SFVDVEIVDVFTNSLRGKFIRGEDEMDLRRDLRPSDILAKHKKDDDLGVTQFIP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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