Request QuoteCatalog Number: xP386371DUTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386371DUTYeast1mgQuote
EP386371DUTE. coli1mgQuote
BP386371DUTBaculovirus200ugQuote
MP386371DUTMammalian Cell200ugQuote

Protein Information

SpeciesClostridium thermocellum (strain ATCC 27405 / DSM 1237)
UniProt IDA3DDI9
Gene NamemiaB; Locus:Cthe_0783
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-480
Expression Tag6xHis
Purity>90%
AA SequenceMSKGTRDRKNIYVSPEEMARQQRFIDEIKELNYRKEVKTGKKKLYCLNTFGCQMNEHDSE KLAGMLAEMGYAETDNVNESDLVIYNTCCVRENAELKVYGHLGMLKPLKNQKPDLVIAVC GCMMQQPEVVEHIKKTYSHVDLIFGTHNLYKFPELLYSAMDSQTTVVDVWDCDGQIAENV AIERKDGVKAWVTVMYGCNNFCTYCIVPYVRGRERSRSMDDILEEVRMLGRQGFKEITLL GQNVNSYGKDIGDGTSFAELIREVNKIPGIERIRFTTSHPKDLSDDLIYAMRDCEKVCEH LHLPFQAGSTRILKLMNRKYTKEDYINLVAKIKENIPDIALTTDIIVGFPGETEEDFSDT LDILEKVRFDNAYTFLYSKRTGTPAAKMEDQVPEEVKKERFQRLLETQNRISKEINDTFL GKVVEVLVEGVSKTNDKIFTGRTRGNKVVNFEADASLIGKLVNVRINTVKTWSLEGSIVR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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