Request QuoteCatalog Number: xP371074MVXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP371074MVXYeast1mgQuote
EP371074MVXE. coli1mgQuote
BP371074MVXBaculovirus200ugQuote
MP371074MVXMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium smegmatis (strain ATCC 700084 / mc (2) 155)
UniProt IDA0QVX5
Gene NamemiaB; Locus:MSMEG_2729
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMVTRDETAEAEGLRPAVGSSRVRTYQVRTYGCQMNVHDSERLSGLLESAGYQRAAEGTDA DIVVFNTCAVRENADNKLYGNLSHLAPRKQADPNMQIAVGGCLAQKDRDAVLRRAPWVDV VFGTHNIGSLPTLLERARHNREAQVEIVEALQEFPSALPASRESAYAAWVSISVGCNNTC TFCIVPSLRGKEVDRRPGDILAEVQSLVDQGVLEITLLGQNVNAYGVSFADPELPRDRGA FAKLLRACGGIDGLERVRFTSPHPAEFTDDVIEAMAATPNVCPTLHMPLQSGSDRILKAM RRSYRAERFLGIIDKVRAAIPHAAITTDLIVGFPGETEEDFQATLDVVEQARFAGAFTFQ YSKRPGTPAADMPDQLPKSVVTERYQRLIELQERISLEQNREQVGRAVELLVATGEGRKD ASTARMSGRARDGRLVHFLPGDNEIRPGDIVTTTVTGAAPHHLIADAPIIERRRTRAGDA HAAGQKPRTGVGLGMPRIGAPAPSATAEGCGC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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